MCD is a kind of amino acid feed widely used in nonalcoholic fatty liver model of rats and mice. Due to the lack of choline and methionine that can be converted into choline in the feed, the fat in the liver can not be normally transported to the blood, so it accumulates in the liver cells and intercellular spaces to form lipid droplets, and then forms fatty liver. MCD induced nonalcoholic fatty liver has the characteristics of rapid modeling and powerful effect. It can form obvious fatty liver complicated with hepatitis within 5 weeks, and then continue to use it to form liver fibrosis and cirrhosis. Its disadvantage is the lack of methionine. As an essential amino acid, it can not synthesize protein without methionine. Animals will gradually lose weight, become worse and die slowly. MCD is often used to induce acute nonalcoholic fatty liver. Its control feed is methionine choline sufficient feed MCS.
(small knowledge: amino acid feed is characterized by using various amino acids to replace casein in conventional feed to supply proteins required by organisms, so as to achieve accurate control of the level of one or more amino acids)

After years of accumulation, the technical team of Ruidi biology has developed various mature modeling feeds and corresponding animal models, such as
1) Ultra high fat for inducing obesity, type 2 diabetes, hyperlipidemia and insulin antagonism in rats and mice
(60kca l%) purified feed and corresponding animal models;
2) High salt purified feed for inducing hypertension and corresponding animal models;
3) Various high-fat feeds for inducing atherosclerosis and corresponding animal models
4) Amino acid purified feed for inducing acute nonalcoholic fatty liver and corresponding animal model;
5) Purified feed for inducing deficiency or excess of various minerals or vitamins and corresponding animal models,
6) Alcohol feed for inducing alcoholic fatty liver and corresponding animal model;
At present, tens of thousands of feed formulas have been accumulated in our database, which is huge. We can't list all the formulas. If you have relevant needs, please contact us. We can recommend mature experimental methods or customize appropriate formulas according to your requirements. We can provide all one-stop solutions.
Now I will elaborate on the specific process of customized feed. If you have any of the following requirements, please let us know.
(1) Special requirements for the feed formula of laboratory animals
(2) Unusual species or strains of laboratory animals
(3) Drugs or compounds need to be pre added to the feed
(4) Special requirements for feed hardness or feed properties (powder / granule / paste / jelly)
(5) Other disease model feed

Feed customization process

According to different situations, the customization process of experimental animal feed is also different. One is that the researchers have demonstrated the feed formula, which needs customized production; The other is that the researcher has no feed formula, or cannot determine the rationality of the formula.

Existing feed formula

(1) The researcher provides the feed formula and relevant information, including the purpose of the experiment and the use of experimental animals.
(2) The Ruidi biotechnology team reviews the rationality of the formula and gives feedback.
(3) After both parties determine the formula, the production department of our company starts to organize production and deliver it to the researcher after completion.

No feed formula or uncertain formula

(1) The researcher shall provide relevant information, including but not limited to the variety / strain of the experimental animal, the purpose of the experiment and the use of the experimental animal.
(2) The Ruidi biotechnology team designs the feed formula according to the effective information, and further consult the literature related to this study if necessary to understand whether there are similar feed formulas in the internationally published papers.
(3) The technical personnel of our company made detailed description on the formula and exchanged views with the researchers.
(4) After the formula is determined, the production department of our company starts to organize production and deliver it to the researcher after completion.

Instructions for feed customization

The customization process of experimental animal feed is not simple, which involves a series of work, such as formula design, procurement of special raw materials, organization of production, etc. Therefore, the following matters should be paid attention to before feed Customization:
(1) Tell you the level of the animal room. Is it normal, clean or SPF?
(2) To confirm the customized feed quantity, the quantity of experimental animals and the feed shelf life shall be considered. If there is too much customization, it will cause waste; If the customized quantity is too small, it will cause repeated production and increase the feed cost.
(3) Inform the experimental group status as much as possible, and the Ruidi biotechnology team will help you find problems and provide suggestions, so as to more accurately count the number of animals in each group.
(4) Since no preservative is added to the feed of laboratory animals, the shelf life of the feed is usually short. Therefore, attention should be paid to the control of storage temperature, otherwise it should be used as soon as possible.
The research and development of experimental animal feed involves many disciplines. We have a team of doctors and masters in nutrition and animal feed science to provide you with technical support, carefully understand your needs for experimental animal models and feed ingredients, and then provide feed formula suggestions according to the scientific literature. Once all feed formulas are confirmed, we promise to complete production and deliver within 5-10 working days.