High sodium diet is usually used to study diet induced hypertension, because increasing circulating sodium level will make cells release water molecules (due to osmotic pressure), thus increasing the pressure on the blood vessel wall and gradually forming hypertension. There are two commonly used high sodium feeds, 4% and 8% sodium chloride feeds. According to different basic feeds, they can be divided into AIN-76 series high sodium feeds and ain-93 series high sodium feeds. Both of the two series of feeds can achieve similar effects.
Hypertension is a disease controlled by multiple genes. At present, the preferred small animal model for hypertension research is rats. Commonly used strains include selectively bred homozygous hypertensive rats (such as SHR and Dahl salt sensitive rats) and outbred strains (such as Sprague Dawley). SHR rats spontaneously increase hypertension in the early life (5-6 weeks) and eventually develop into hypertension. Because its peripheral resistance and normal cardiac output are similar to human hypertension, this strain is considered to be a good model for the study of essential hypertension. It usually takes 4-6 weeks for Dahl salt sensitive rats to produce hypertension when using 8% high salt diet. However, individual weaned Dahl SS rats can rapidly produce hypertension (> 180 mmHg) in just 2 weeks when feeding.
Relatively speaking, mice are not widely used in hypertension modeling. Even with 8% high salt purified feed, inbred mice such as C57BL / 6 can detect elevated blood pressure, but this process takes several months. However, because wild-type mice are easy to obtain and economical, many researchers choose to induce hypertension in reality. At this time, the best choice is 8% high sodium diet.

After years of accumulation, the technical team of Ruidi biology has developed various mature modeling feeds and corresponding animal models, such as
1) Ultra high fat for inducing obesity, type 2 diabetes, hyperlipidemia and insulin antagonism in rats and mice (60kca l%) purified feed and corresponding animal models;
2) High salt purified feed for inducing hypertension and corresponding animal models;
3) Various high-fat feeds for inducing atherosclerosis and corresponding animal models
4) Amino acid purified feed for inducing acute nonalcoholic fatty liver and corresponding animal model;
5) Purified feed for inducing deficiency or excess of various minerals or vitamins and corresponding animal models,
6) Alcohol feed for inducing alcoholic fatty liver and corresponding animal model;
At present, tens of thousands of feed formulas have been accumulated in our database, which is huge. We can't list all the formulas. If you have relevant needs, please contact us. We can recommend mature experimental methods or customize appropriate formulas according to your requirements. We can provide all one-stop solutions.
Now I will elaborate on the specific process of customized feed. If you have any of the following requirements, please let us know.
(1) Special requirements for the feed formula of laboratory animals
(2) Unusual species or strains of laboratory animals
(3) Drugs or compounds need to be pre added to the feed
(4) Special requirements for feed hardness or feed properties (powder / granule / paste / jelly)
(5) Other disease model feed

Feed customization process

According to different situations, the customization process of experimental animal feed is also different. One is that the researchers have demonstrated the feed formula, which needs customized production; The other is that the researcher has no feed formula, or cannot determine the rationality of the formula.

Existing feed formula

(1) The researcher provides the feed formula and relevant information, including the purpose of the experiment and the use of experimental animals.
(2) The Ruidi biotechnology team reviews the rationality of the formula and gives feedback.
(3) After both parties determine the formula, the production department of our company starts to organize production and deliver it to the researcher after completion.

No feed formula or uncertain formula

(1) The researcher shall provide relevant information, including but not limited to the variety / strain of the experimental animal, the purpose of the experiment and the use of the experimental animal.
(2) The Ruidi biotechnology team designs the feed formula according to the effective information, and further consult the literature related to this study if necessary to understand whether there are similar feed formulas in the internationally published papers.
(3) The technical personnel of our company made detailed description on the formula and exchanged views with the researchers.
(4) After the formula is determined, the production department of our company starts to organize production and deliver it to the researcher after completion.

Instructions for feed customization

The customization process of experimental animal feed is not simple, which involves a series of work, such as formula design, procurement of special raw materials, organization of production, etc. Therefore, the following matters should be paid attention to before feed Customization:
(1) Tell you the level of the animal room. Is it normal, clean or SPF?
(2) To confirm the customized feed quantity, the quantity of experimental animals and the feed shelf life shall be considered. If there is too much customization, it will cause waste; If the customized quantity is too small, it will cause repeated production and increase the feed cost.
(3) Inform the experimental group status as much as possible, and the Ruidi biotechnology team will help you find problems and provide suggestions, so as to more accurately count the number of animals in each group.
(4) Since no preservative is added to the feed of laboratory animals, the shelf life of the feed is usually short. Therefore, attention should be paid to the control of storage temperature, otherwise it should be used as soon as possible.
The research and development of experimental animal feed involves many disciplines. We have a team of doctors and masters in nutrition and animal feed science to provide you with technical support, carefully understand your needs for experimental animal models and feed ingredients, and then provide feed formula suggestions according to the scientific literature. Once all feed formulas are confirmed, we promise to complete production and deliver within 5-10 working days.